PurposeExpresses CRISPR base editor in protoplasts
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98161||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Promoter Ubi
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Bsp1407I (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAPOBEC1, XTEN, dCas9 (D10A and H840A) and UGI sequences were codon-optimized for cereal plants, and synthesized commercially (GenScript, Nanjing, China).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pdCas9-PBE was a gift from Caixia Gao (Addgene plasmid # 98161 ; http://n2t.net/addgene:98161 ; RRID:Addgene_98161)
For your References section:Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. 10.1038/nbt.3811 PubMed 28244994