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pHBS838 TDP43 RRM-GFP
(Plasmid #98249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 98249-D50 50 μg of DNA in Tris buffer $495
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DNA 98249-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS-Dest
  • Total vector size (bp) 5680
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARDBP
  • Alt name
    TAR DNA binding protein
  • Alt name
    TDP-43
  • Alt name
    TDP43
  • Species
    H. sapiens (human)
  • GenBank ID
    23435
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Promoter CMV
  • Tag / Fusion Protein
    • Internal GFP to replace RRM domains

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATTTAGGTGACACTATAG
  • 3′ sequencing primer TAATACGACTCACTATAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original, unmodified TARDBP clone was derived from Addgene #27462

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBS838 TDP43 RRM-GFP was a gift from Rajat Rohatgi (Addgene plasmid # 98249 ; http://n2t.net/addgene:98249 ; RRID:Addgene_98249)

  • For your References section:

    In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes. Schmidt HB, Rohatgi R. Cell Rep. 2016 Aug 2;16(5):1228-36. doi: 10.1016/j.celrep.2016.06.088. Epub 2016 Jul 21. 10.1016/j.celrep.2016.06.088 PubMed 27452472
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