pHBS838 TDP43 RRM-GFP
(Plasmid #98249)

• Purpose: TDP43 RRM-GFP reporter construct
• Depositing Lab: Rajat Rohatgi
• Publication: Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi: 10.1016/j.celrep.2016.06.088. Epub 2016 Jul 21.

Backbone

• Vector backbone: pCS-Dest
• Total vector size (bp): 5680
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TARDBP
• Alt name: TAR DNA binding protein
• Alt name: TDP-43
• Alt name: TDP43
• Species: H. sapiens (human)
• GenBank ID: 23435
• Entrez Gene: TARDBP (a.k.a. ALS10, TDP-43)
• Promoter: CMV
• Tag / Fusion Protein:
  • Internal GFP to replace RRM domains

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATTTAGGTGACACTATAG
• 3′ sequencing primer: TAATACGACTCACTATAG

Resource Information

• Supplemental Documents:
  • pHBS838 TDP43 RRM-GFP.gbk
  • Sequence
• A portion of this plasmid was derived from a plasmid made by: Original, unmodified TARDBP clone was derived from Addgene #27462

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry