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pTNFR1-tdEOS
(Plasmid #98273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98273 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 98273-D50 50 μg of DNA in Tris buffer $495
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DNA 98273-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3-Flag1-tdEosFP
  • Backbone manufacturer
    MoBiTec
  • Vector type
    Mammalian Expression
  • Selectable markers
    tdEos

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNFR1
  • Alt name
    TNFRSF1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1392
  • Entrez Gene
    TNFRSF1A (a.k.a. CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • tdEos (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

fusion protein: TNFR1-tdEosfp

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTNFR1-tdEOS was a gift from Mike Heilemann (Addgene plasmid # 98273 ; http://n2t.net/addgene:98273 ; RRID:Addgene_98273)

  • For your References section:

    TNF-alpha influences the lateral dynamics of TNF receptor I in living cells. Heidbreder M, Zander C, Malkusch S, Widera D, Kaltschmidt B, Kaltschmidt C, Nair D, Choquet D, Sibarita JB, Heilemann M. Biochim Biophys Acta. 2012 Oct;1823(10):1984-9. doi: 10.1016/j.bbamcr.2012.06.026. Epub 2012 Jun 27. 10.1016/j.bbamcr.2012.06.026 PubMed 22749881
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