CytERM-mTurquoise2
(Plasmid #98833)

• Purpose: In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
• Depositing Lab: Dorus Gadella
• Publication: Gadella lab plasmids (unpublished)

Backbone

• Vector backbone: pEGFP-N1
  (Search Vector Database)
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CytERM
• Species: Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 29_Cyterm_mTq2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry