pRRG882-F-spondin
(Plasmid #99096)

• Purpose: dsRNA and riboprobe synthesis for Schmidtea mediterranea F-spondin
• Depositing Lab: Phillip Newmark
• Publication: Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.

Backbone

• Vector backbone: pJC53.2
• Backbone manufacturer: Phillip Newmark (Addgene plasmid # 26536)
• Backbone size w/o insert (bp): 3240
• Vector type: T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: F-spondin in situ probe
• Alt name: KY024329.1 (Genbank)
• Alt name: dd_Smed_v6_2400_0_1
• Species: Schmidtea mediterranea
• Insert Size (bp): 994

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Eam1105I (destroyed during cloning)
• 3′ cloning site: Eam1105I (destroyed during cloning)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning primers: F-AAACAAAAGAGCATGGTTCA, R-AATCTTGCAAACATTCGAGA

Note: Only 143 base pairs from the insert align with KY024329.1. The depositor has confirmed this does not impact plasmid function.