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(Plasmid #99240)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99240 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 8220
  • Modifications to backbone
    HKII cDNA (cloned from rat AS-30D hepatoma) inserted in MCS of vector between EcoRI and XbaI sites. A Kozak sequence was for enhanced translation initiation.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Hexokinase II
  • Alt name
  • Alt name
    Type II hexokinase
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    Four silent mutations; P (115)L, A (192) V, F (782) S, L (854) P
  • GenBank ID
  • Entrez Gene
  • Promoter CMV I.E.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAG
  • 3′ sequencing primer ATTAACCCTCACTAAAGGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's QC result found 4 additional mutations (V192A, V378A, M555V, and E699G), that are unexpected; however, the depositor notes that they are away from the catalytic site (and the catalytic site is active).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-HKIImut was a gift from Peter Pedersen (Addgene plasmid # 99240 ; ; RRID:Addgene_99240)
  • For your References section:

    Glucose catabolism in cancer cells. The type II hexokinase promoter contains functionally active response elements for the tumor suppressor p53. Mathupala SP, Heese C, Pedersen PL. J Biol Chem. 1997 Sep 5;272(36):22776-80. PubMed 9278438