HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
(Plasmid #99342)

• Purpose: Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD
• Depositing Lab: Peter Wright
• Publication: Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10.1038/emboj.2009.30. Epub 2009 Feb 12.

Backbone

• Vector backbone: pET22b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5493
• Total vector size (bp): 6208
• Modifications to backbone: introduced second RBS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: STAT1 (710-750)
• Alt name: isgf3
• Species: H. sapiens (human)
• Insert Size (bp): 715
• GenBank ID: NM_007315
• Entrez Gene: STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
• Promoter: T7
• Tag / Fusion Protein:
  • His6GB1 (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: CBP (1764-1855)
• Alt name: crebbp
• Species: M. musculus (mouse)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry