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HisGB1-Hif1a(776-826)_FIH
(Plasmid #99508)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99508 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 7003
  • Modifications to backbone
    introduced second RBS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Hif1a (776-826)
  • Alt name
    Hif1a
  • Species
    H. sapiens (human)
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Promoter T7
  • Tag / Fusion Protein
    • His6GB1 (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    FIH1
  • Alt name
    HIF1AN
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_017902

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisGB1-Hif1a(776-826)_FIH was a gift from Peter Wright (Addgene plasmid # 99508 ; http://n2t.net/addgene:99508 ; RRID:Addgene_99508)
  • For your References section:

    Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes. Sugase K, Landes MA, Wright PE, Martinez-Yamout M. Protein Expr Purif. 2008 Feb;57(2):108-15. Epub 2007 Nov 1. 10.1016/j.pep.2007.10.018 PubMed 18054500