|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99529||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepRS306 derivative
Vector typePCR template for yeast tagging cassette
Growth in Bacteria
Copy numberHigh Copy
Alt nameAID (Auxin-inducible degron)
SpeciesA. thaliana (mustard weed)
Entrez GeneAXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)
- Promoter CUP1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CGTACGCTGCAGGTCGAC
- 3′ sequencing primer GCTGGTACCGAGCTCTGG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe parent vector encoding AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHyg-PCUP1-AID*(N) was a gift from Helle Ulrich (Addgene plasmid # 99529 ; http://n2t.net/addgene:99529 ; RRID:Addgene_99529)