PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99536||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGietz & Sugino (1988) Gene 74:527-34
- Backbone size w/o insert (bp) 5741
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)991
Entrez GeneSMT3 (a.k.a. YDR510W)
- Promoter CUP1
/ Fusion Protein
- 7xHis (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer M13fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YEp181-CUP1-His-Smt3 was a gift from Helle Ulrich (Addgene plasmid # 99536 ; http://n2t.net/addgene:99536 ; RRID:Addgene_99536)
For your References section:In vivo detection and characterization of sumoylation targets in Saccharomyces cerevisiae. Ulrich HD, Davies AA. Methods Mol Biol. 2009;497:81-103. doi: 10.1007/978-1-59745-566-4_6. 10.1007/978-1-59745-566-4_6 PubMed 19107412