Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

YIp211-P30-His-pol30(K127/164R)-3'
(Plasmid #99549)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99549 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YIplac211
  • Backbone manufacturer
    Gietz & Sugino (1988) Gene 74:527-34
  • Backbone size w/o insert (bp) 3797
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POL30
  • Alt name
    PCNA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2502
  • Mutation
    K127R, K164R
  • Entrez Gene
    POL30 (a.k.a. YBR088C)
  • Promoter POL30
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer M13fwd
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIp211-P30-His-pol30(K127/164R)-3' was a gift from Helle Ulrich (Addgene plasmid # 99549 ; http://n2t.net/addgene:99549 ; RRID:Addgene_99549)
  • For your References section:

    Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD. Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36. 10.1007/978-1-61779-998-3_36 PubMed 22941627