Tre-Tight ifgMosaic Donor vector (LH121)
(Plasmid #99630)

• Purpose: To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics
• Depositing Lab: Rui Benedito
• Publication: Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031.

Backbone

• Vector backbone: pUC18
• Vector type: Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N-PhiM
• Species: Synthetic
• Mutation: (Please see depositor comments below)

Resource Information

• Supplemental Documents:
  • LH121 GB

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a 2bp insertion at start of FRT site was found during Addgene's Quality Control process. The depositing lab has noted that the FRT site is now non functional in the presence of FlpO enzyme. However in the case of this plasmid, the FRT site is not required for the plasmid to be functional, since no FlipOUT (FlpO) is going to be used.