pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP
(Plasmid
#99731)
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Purposeoverexpression of PDGFRa and CopGFP
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Depositing Lab
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Depositing OrganizationHangzhou Normal University
Located in China -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 99731-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 99731-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDH-CMV-MCS-EF1-nlsCopGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePdgfra
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SpeciesM. musculus (mouse)
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Insert Size (bp)3340
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Entrez GenePdgfra (a.k.a. CD140a, Pdgfr-2)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 99731-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 99731-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP was a gift from Mengsheng Qiu (Addgene plasmid # 99731 ; http://n2t.net/addgene:99731 ; RRID:Addgene_99731) -
For your References section:
Tmeff2 is expressed in differentiating oligodendrocytes but dispensable for their differentiation in vivo. Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M. Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1. 10.1038/s41598-017-00407-1 [pii] PubMed 28336932
