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(Plasmid #99843)


Item Catalog # Description Quantity Price (USD)
Plasmid 99843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4698
  • Total vector size (bp) 7071
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Optimal growth in Stbl3 at 30°C (use Carbenicillin).
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Channelrhodopsin-2 H134R mutant
  • Alt name
    tandem dimer Tomato fluorescent protein
  • Species
    Chlamydomonas reinhardtii (modified)
  • Insert Size (bp)
  • Mutation
    ChR2(H134R), carrying a single point mutation at position H134, the native histidine residue is replaced by a more basic arginine.
  • GenBank ID
    EF474017.1 AY678269.1
  • Promoter human synapsin I promoter
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ggagccgtggcggcaccggtagtagcaGTGAGCAAGGGCG
  • 3′ sequencing primer gctttgtacggaattcTTACTTGTACAGCTCGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone vector:

Edward Boyden
Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Scott Sternson
Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Syn-ChR-tdTomato was a gift from Gordon Shepherd (Addgene plasmid # 99843 ; ; RRID:Addgene_99843)