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Ca -independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of E. coli.
Hirakawa H, Ishikawa S, Nagamune T
Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64983pASKt15C+SrtAExpresses the wild type of Staphylococcus aureus sortase A in E. coli
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64984pASKt15C+SrtA2Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli
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64985pASKt15C+SrtA5Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
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64986pASKt15C+SrtA7Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
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65017pASKt15C+SrtAwoHExpresses the wild type of Staphylococcus aureus sortase A in E. coli
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65018pASKt15C+SrtA2woHExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli
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65019pASKt15C+SrtA5woHExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus
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65020pASKt15C+SrtA7woHExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
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