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Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.

Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65591AP56-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
65593AP56-6co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
65594AP56-7co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
65596AP56-8co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
65597AP54-3co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
65599AP54-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
66085APq5271co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
66086APq5210co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
66087AP303-3co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
66088AP303-4co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
66089AP332-2co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
66090AP332-3co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
66091AP332-4co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
66092AP324-2co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66093AP324-3co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66094AP324-4co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66095AP334-2co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66096AP334-5co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66097AP334-6co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
66099APCSD 53co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2
66100APCSD 54co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Antibodies from Article