Quantifying cellular capacity identifies gene expression designs with reduced burden.
Ceroni F, Algar R, Stan GB, Ellis T
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66073 | Burden Monitor lambda version | The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells |
| 66076 | pInt-ts | The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version |
| 66077 | pAH123 | The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version |