Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
BMC Biotechnol. 2015 May 22;15(1):33.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63596 | pX330A_dCas9-1x2 | Expresses dCas9 and gRNA |
| 63597 | pX330A_dCas9-1x3 | Expresses dCas9 and gRNA |
| 63598 | pX330A_dCas9-1x4 | Expresses dCas9 and gRNA |
| 63599 | pX330A_dCas9-1x5 | Expresses dCas9 and gRNA |
| 63600 | pX330A_dCas9-1x6 | Expresses dCas9 and gRNA |
| 63601 | pX330A_dCas9-1x7 | Expresses dCas9 and gRNA |
| 63602 | pX330A_FokI-1x2 | Expresses FokI-dCas9 and gRNA |
| 63603 | pX330A_FokI-1x3 | Expresses FokI-dCas9 and gRNA |
| 63604 | pX330A_FokI-1x4 | Expresses FokI-dCas9 and gRNA |
| 63605 | pX330A_FokI-1x5 | Expresses FokI-dCas9 and gRNA |
| 63606 | pX330A_FokI-1x6 | Expresses FokI-dCas9 and gRNA |
| 63607 | pX330A_FokI-1x7 | Expresses FokI-dCas9 and gRNA |