PurposeExpresses FokI-dCas9 and gRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63604||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC ori vector
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. pyogenes and F. okeanokoites
- Promoter CBh
- Cloning method Unknown
- 5′ sequencing primer T7 (TAATACGACTCACTATAGGG) (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX330A_FokI-1x4 was a gift from Takashi Yamamoto (Addgene plasmid # 63604 ; http://n2t.net/addgene:63604 ; RRID:Addgene_63604)
For your References section:Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T. BMC Biotechnol. 2015 May 22;15(1):33. 10.1186/s12896-015-0144-x [pii] PubMed 25997509