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Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
BMC Biotechnol. 2015 May 22;15(1):33.
PubMed

Plasmids from Article

ID Plasmid Purpose  
63596pX330A_dCas9-1x2Expresses dCas9 and gRNA
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63597pX330A_dCas9-1x3Expresses dCas9 and gRNA
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63598pX330A_dCas9-1x4Expresses dCas9 and gRNA
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63599pX330A_dCas9-1x5Expresses dCas9 and gRNA
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63600pX330A_dCas9-1x6Expresses dCas9 and gRNA
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63601pX330A_dCas9-1x7Expresses dCas9 and gRNA
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63602pX330A_FokI-1x2Expresses FokI-dCas9 and gRNA
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63603pX330A_FokI-1x3Expresses FokI-dCas9 and gRNA
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63604pX330A_FokI-1x4Expresses FokI-dCas9 and gRNA
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63605pX330A_FokI-1x5Expresses FokI-dCas9 and gRNA
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63606pX330A_FokI-1x6Expresses FokI-dCas9 and gRNA
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63607pX330A_FokI-1x7Expresses FokI-dCas9 and gRNA
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