PurposeExpresses dCas9 and gRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63597||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepUC ori vector
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namehumanized S. pyogenes dCas9
Insert Size (bp)4272
- Promoter CBh
/ Fusion Protein
- 3xFLAG (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer T7 (TAATACGACTCACTATAGGG) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX330A_dCas9-1x3 was a gift from Takashi Yamamoto (Addgene plasmid # 63597 ; http://n2t.net/addgene:63597 ; RRID:Addgene_63597)
For your References section:Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T. BMC Biotechnol. 2015 May 22;15(1):33. 10.1186/s12896-015-0144-x [pii] PubMed 25997509