Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Floe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66818pCCL-PGK-SPdCas9-BFP-DNMT1dCas9 fused to BFP and the human DNMT1 catalytic domain
Loading...
66819pCCL-PGK-SPdCas9-BFP-DNMT3AdCas9 fused to BFP and the human DNMT3A catalytic domain
Loading...
66820pCCL-PGK-SPdCas9-BFP-DNMT3BdCas9 fused to BFP and the human DNMT3B catalytic domain
Loading...
66821pCCL-PGK-SPdCas9-BFP-EGFPdCas9 fused to BFP and EGFP
Loading...
71213pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)dCas9 fused to BFP and the human DNMT3A catalytically inactive domain
Loading...
71214pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)dCas9 fused to BFP and the human DNMT3B catalytically inactive domain
Loading...
71215pLenti-EF1a-SPdCas9-EGFP-2A-BlastdCas9 fused to EGFP
Loading...
71216pLenti-EF1a-SPdCas9-DNMT3A-2A-BlastdCas9 fused to human DNMT3A catalytic domain
Loading...
71217pLenti-EF1a-SPdCas9-DNMT3B-2A-BlastdCas9 fused to human DNMT3B catalytic domain
Loading...
71218pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-BlastdCas9 fused to the human DNMT3A catalytically inactive domain
Loading...
71219pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-BlastdCas9 fused to the human DNMT3B catalytically inactive domain
Loading...