Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and its Substrate RNA Splicing Factor SF3B2.
Hadjikyriacou A, Yang Y, Espejo A, Bedford MT, Clarke SG
J Biol Chem. 2015 May 15. pii: jbc.M115.659433.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
67607GST-PRMT9 WTbacterial expression of wild type human PRMT9
67608GST-PRMT9 L182A/D183A/I184A/G185Abacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant
67609GST-PRMT9 D258G mutantbacterial expression of GST-PRMT9 D258G Double E loop mutant
67610GST-PRMT9 G260E mutantbacterial expression of GST-PRMT9 G260E Double E loop mutant
67611GST-PRMT9 deltaN (aa 21-350)bacterial expression of GST-PRMT9 missing residues 21-350
67612GST-PRMT9 deltaC 350 (stop)bacterial expression of GST-PRMT9 missing residues 351-845
67614GST-PRMT9 deltaTPR mutantbacterial expression of GST-PRMT9 missing residues 21-139
67615GST-SF3B2 wild type aa 401-550 fragmentbacterial expression of wild type GST-SF3B2 fragment (401-550)
79674GST-SF3B2 R508K aa 401-550 fragmentbacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation
79675GFP-PRMT9mammalian expression of GFP-PRMT9
79676GFP-PRMT9 L182A/D183A/I184A/G185Amammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Antibodies from Article