High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
Paix A, Folkmann A, Rasoloson D, Seydoux G
Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70047 | AP568-2 | co-expression of Cas9 and crRNA 589 target sequence |
| 70048 | AP568-3 | co-expression of Cas9 and crRNA 589 target sequence |
| 70049 | AP582-2 | Expresses Ollas::mCherry::linker::H2B::V5 in bacteria |
| 70050 | AP682-1 | Expresses TEV::eGFP::myc::3Xflag in bacteria |
| 70051 | AP625-1 | Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria |
| 71277 | AP575-1 | Expresses 3Xflag::tagRFP::myc in bacteria |
| 71278 | AP588-1 | Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria |