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Insights into Ubiquitination from the Unique Clamp-like Binding of the RING E3 AO7 to the E2 UbcH5B.
Li S, Liang YH, Mariano J, Metzger MB, Stringer DK, Hristova VA, Li J, Randazzo PA, Tsai YC, Ji X, Weissman AM
J Biol Chem. 2015 Dec 18;290(51):30225-39. doi: 10.1074/jbc.M115.685867. Epub 2015 Oct 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
137840pCDNA3.1+ HA-hAO7N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells
137842pGEX6p1 AO7RGST tagged AO7 encoding aa 126-216 for expression in bacteria
137843pGEX6p1 AO7REGST tagged AO7 encoding aa 126-258 for expression in bacteria
139318pETDUET-GST AO7RE ∆238-245GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria
139319pETDUET-GST AO7RE I137AGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria
139427pETDUET-GST AO7RE P199AGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria
139428pETDUET-GST AO7RE Y165AGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria
213935pETDUET-GST AO7REwtGST tagged AO7 encoding aa 126-258 for expression in bacteria

Antibodies from Article