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Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress.
Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74374pX462-hPRKAA1-gRNA_AgRNA_A to knockout human AMPK alpha 1 using Cas9n
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74375pX462-hPRKAA1-gRNA_BgRNA_B to knockout human AMPK alpha 1 using Cas9n
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74376pX462-hPRKAA2-gRNA_AgRNA_A to knockout human AMPK alpha 2 using Cas9n.
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74377pX462-hPRKAA2-gRNA_BgRNA_B to knockout human AMPK alpha 2 using Cas9n
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74378pCDNA3 Flag MFF iso5 WTFlag tagged human MFF isoform 5
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74379pLenti.PGK.blast-Flag-MFF-WTLentiviral expression vector containing flag tagged WT MFF isoform 5
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74380pLenti.PGK.blast-Flag-MFF-S155,172ALentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutant
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74381pCDNA3 Flag MFF iso5 S155AFlag tagged human S155A MFF isoform 5 mutant
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74382pCDNA3 Flag MFF iso5 S172AFlag tagged human S172A MFF isoform 5 mutant
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74383pCDNA3 Flag MFF iso5 S155,172AFlag tagged human S155,172A MFF isoform 5 mutant
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74384pQCXIB Flag MFF WTRetroviral expression vector containing Flag tagged WT MFF isoform 5
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74385pQCXIB Flag MFF S155ARetroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant
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74386pQCXIB Flag MFF S172ARetroviral expression vector containing Flag tagged S172A MFF isoform 5 mutant
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74387pQCXIB Flag MFF S155,172ARetroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant
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74388pcDNA3 Flag MFF iso1 WTFlag tagged human MFF isoform 1
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74389pcDNA3 Flag MFF iso2 WTFlag tagged human MFF isoform 2
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74390pcDNA3 Flag MFF iso4 WTFlag tagged human MFF isoform 4
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74391pcDNA3 Flag MFF iso1 S172AFlag tagged human S172A MFF isoform 1 mutant
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74392pcDNA3 Flag MFF iso2 S172AFlag tagged human S172A MFF isoform 2 mutant
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74393pcDNA3 Flag MFF iso4 S172AFlag tagged human S172A MFF isoform 4 mutant
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74442pLenti.PGK.blast-Flag-MFF-S155,172DLentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant
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74443pLenti.PGK.blast-Flag-MFF-S155,172ELentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant
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74444pLenti.PGK.blast-Renilla_LuciferaseControl lentiviral vector expressing Renilla luciferase
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74445pQCXIB Firefly_LuciferaseControl retroviral vector expressing firefly luciferase
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74446pLenti.CMV/TO_PRKAA1Lentiviral expression vector encoding untagged WT human AMPK alpha 1
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74447pLenti.CMV/TO_PRKAA2Lentiviral expression vector encoding untagged WT human AMPK alpha 2
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