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LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate.
Belluzzi E, Gonnelli A, Cirnaru MD, Marte A, Plotegher N, Russo I, Civiero L, Cogo S, Carrion MP, Franchin C, Arrigoni G, Beltramini M, Bubacco L, Onofri F, Piccoli G, Greggio E
Mol Neurodegener. 2016 Jan 13;11(1):1. doi: 10.1186/s13024-015-0066-z.
PubMed

Plasmids from Article

ID Plasmid Purpose  
74924pcDNA3-Flag-NSFMammalian expression of human NSF full-length (744 aa)
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74936pcDNA3-Flag-NSF/T645AMammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)
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74937pcDNA3-Flag-NSF/T646AMammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)
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74938pcDNA3-Flag-NSF/S647AMammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)
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Recombinant Antibodies from Article