Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
Vancraenenbroeck R, Kunzelmann S, Webb MR
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. PubMed Article
Vancraenenbroeck R, Kunzelmann S, Webb MR
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78206 | pTEV5_ RpMatB_2 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding |
| 78207 | pTEV5_ RpMatB_3 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding |
| 78208 | pTEV5_ RpMatB_4 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding |