4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation.
Igreja C, Peter D, Weiler C, Izaurralde E
Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790. PubMed Article
Igreja C, Peter D, Weiler C, Izaurralde E
Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79261 | pAc5.1B-EGFP-Dm4E-T | Expresses EGFP-tagged Dm4E-T in S2 cells |
| 79408 | pAc5.1B-EGFP-DmThor-V5His6 | Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells |
| 146149 | pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D | Insect Expression of Dm4E-T_1-510 |
| 146150 | pAc5.1B-lambdaN-HA-Dm4E-T_D | Insect Expression of Dm4E-T |
| 146151 | pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D | Insect Expression of Dm4E-T_1-510 |
| 146152 | pAc5.1B-lambdaN-HA-Dm4E-T_1-510-Y10AL15A-V5His6_D | Insect Expression of Dm4E-T_1-510-Y10AL15A |
| 146153 | pAc5.1B-lambdaN-HA-Dm4E-T-Y10AL15A_D | Insect Expression of Dm4E-T-Y10AL15A |
| 146398 | pAc5.1B-lambdaN-HA-DmThor-V5His6_G | Insect Expression of DmThor |
| 146577 | pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I | Insect Expression of DmeIF4Ga |
| 147100 | pAc5.1B-EGFP-DmCup-4Ala-mut_O | Insect Expression of DmCup-4Ala-mut |
| 147101 | pAc5.1B-EGFP-DmCup-del580-600_O | Insect Expression of DmCup-del580-600 |
| 147102 | pAc5.1B-EGFP-DmCup-L379AL383A_O | Insect Expression of DmCup-L379AL383A |
| 147103 | pAc5.1B-EGFP-DmCup-Y342AL347A_O | Insect Expression of DmCup-Y342AL347A |
| 147575 | pAc5.1B-EGFP-Dm4E-T_739-1010_T | Insect Expression of Dm4E-T_739-1010 |
| 147576 | pAc5.1B-EGFP-Dm4E-T-del739-1010_T | Insect Expression of Dm4E-T-del739-1010 |
| 147577 | pAc5.1B-EGFP-Dm4E-T-NRE90AAA_T | Insect Expression of Dm4E-T-NRE90AAA |
| 147578 | pAc5.1B-EGFP-DmCYFIP1_T | Insect Expression of DmCYFIP1 |
| 147579 | pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmCup_417-1133_T | Insect Expression of Dm4E-T_1-58-DmCup_417-1133 |
| 147580 | pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T | Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666 |
| 147581 | pAc5.1B-lambdaN-HA-Dm4E-T_739-1010_T | Insect Expression of Dm4E-T_739-1010 |
| 147582 | pAc5.1B-lambdaN-HA-Dm4E-T-NRE90AAA_T | Insect Expression of Dm4E-T-NRE90AAA |
| 147583 | pAc5.1B-lambdaN-HA-DmCup_1-417-CG32016-E_T | Insect Expression of DmCup_1-417-CG32016-E |
| 147584 | pAc5.1B-lambdaN-HA-DmCup_1-417-DmeIF4G_686-1666_T | Insect Expression of DmCup_1-417-DmeIF4G_686-1666 |
| 147585 | pAc5.1B-lambdaN-HA-DmCYFIP1_T | Insect Expression of DmCYFIP1 |
| 147586 | pAc5.1B-lambdaN-HA-DmCYFIP1-4xmut_T | Insect Expression of DmCYFIP1-4xmut |
| 147587 | pAc5.1B-lambdaN-HA-DmCYFIP1-K743E_T | Insect Expression of DmCYFIP1-K743E |
| 147588 | pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T | Insect Expression of DmeIF4Ga_578-650-CG32016-E |
| 147589 | pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-DmCup_417-1133_T | Insect Expression of DmeIF4Ga_578-650-DmCup_417-1133 |
| 147590 | pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T | Insect Expression of DmThor-CG32016-E |
| 147591 | pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T | Insect Expression of DmThor-DmCup_417-1133 |
| 147592 | pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T | Insect Expression of DmThor-DmeIF4Ga_687-1666 |
| 147593 | pAc5.1B-EGFP-Dm4E-T-4xmut_T | Insect Expression of Dm4E-T-4xmut |
| 147594 | pAc5.1B-EGFP-Dm4E-T-5xmut_T | Insect Expression of Dm4E-T-5xmut |
| 147595 | pAc5.1B-EGFP-Dm4E-T_1-58_T | Insect Expression of Dm4E-T_1-58 |
| 147596 | pAc5.1B-EGFP-Dm4E-T_1-58-del28-43_T | Insect Expression of Dm4E-T_1-58-del28-43 |
| 147597 | pAc5.1B-EGFP-Dm4E-T_1-58-FWK41AAA_T | Insect Expression of Dm4E-T_1-58-FWK41AAA |
| 147598 | pAc5.1B-EGFP-Dm4E-T_1-58-L36AL39A_T | Insect Expression of Dm4E-T_1-58-L36AL39A |
| 147600 | pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T | Insect Expression of Dm4E-T_1-510-Y10AL15A |
| 147601 | pAc5.1B-EGFP-Dm4E-T-del28-43_T | Insect Expression of Dm4E-T-del28-43 |
| 147602 | pAc5.1B-EGFP-Dm4E-T-del312-356_T | Insect Expression of Dm4E-T-del312-356 |
| 147603 | pAc5.1B-EGFP-Dm4E-T-FWK41AAA_T | Insect Expression of Dm4E-T-FKW41AAA |
| 147604 | pAc5.1B-EGFP-Dm4E-T-L36AL39A_T | Insect Expression of Dm4E-T-L36AL39A |
| 147605 | pAc5.1B-EGFP-Dm4E-T-Y10AL15A_T | Insect Expression of Dm4E-T-Y10AL15A |
| 147606 | pAc5.1B-lambdaN-HA-Dm4E-T-4xmut_T | Insect Expression of Dm4E-T-4xmut |
| 147607 | pAc5.1B-lambdaN-HA-Dm4E-T-5xmut_T | Insect Expression of Dm4E-T-5xmut |
| 147608 | pAc5.1B-lambdaN-HA-Dm4E-T_1-738_T | Insect Expression of Dm4E-T_1-738 |
| 147609 | pAc5.1B-lambdaN-HA-Dm4E-T-del28-43_T | Insect Expression of Dm4E-T-del28-43 |
| 147610 | pAc5.1B-lambdaN-HA-Dm4E-T-del312-356_T | Insect Expression of Dm4E-T-del321-356 |
| 147611 | pAc5.1B-lambdaN-HA-Dm4E-T-FWK41AAA_T | Insect Expression of Dm4E-T-FWK41AAA |
| 147612 | pAc5.1B-lambdaN-HA-Dm4E-T-L36AL39A_T | Insect Expression of Dm4E-T-L36AL39A |
| 147613 | pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T | Insect Expression of DmThor-del76-85 |
| 148025 | pnEA-NpM-DmCup_325-376-GB1_Y | Bacterial Expression of DmCup_325-376 |
| 148026 | pnEA-NpM-DmCUP_325-342-GB1_Y | Bacterial Expression of DmCup_325-342 |
| 148027 | pnEA-NpM-DmCUP_362-376-GB1_Y | Bacterial Expression of DmCup_362-376 |
| 148152 | pAc5.1B-lambdaN-HA-DmThor-del76-84-V5His6_Z | Insect Expression of DmThor-del76-84 |
| 148153 | pAc5.1B-lambdaN-HA-DmThor-YMCLLtoAAAAA_Z | Insect Expression of DmThor-YMCLLtoAAAAA |
| 148154 | pnEA-NvM-DmeIF4G_578-650-GB1_Z | Bacterial Expression of DmeIF4G_578-650 |
| 148156 | pAc5.1B-EGFP-Dm4E-T-LLFWKtoAAAAA_Z | Insect Expression of Dm4E-T-LLFWKtoAAAAA |
| 148157 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z | Insect Expression of Dm4E-T_1-511-del28-43 |
| 148158 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z | Insect Expression of Dm4E-T_1-511-FWKtoAAA |
| 148159 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z | Insect Expression of Dm4E-T_1-511-L36AL69A |
| 148160 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z | Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA |
| 148161 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z | Insect Expression of Dm4E-T_1-511-Y10AL15A |
| 148162 | pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z | Insect Expression of Dm4E-T_1-511-YLLLtoAAAA |
| 148163 | pAc5.1B-lambdaN-HA-DmeIF4E-I96AI112A-siRNAres_Z | Insect Expression of DmeIF4E-I96AI112A |
| 148164 | pAc5.1B-lambdaN-HA-DmeIF4E-W106A-siRNAres_Z | Insect Expression of DmeIF4F-W106A |
| 148165 | pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z | Insect Expression of DmThor-CLL78AAA |
| 148166 | pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z | Insect Expression of DmThor-RGTtoAAA |
| 148167 | pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z | Insect Expression of DmThor-Y54AM59A |
| 148168 | pGex-CS-DmCup_296-425-GB1_Z | Bacterial Expression of DmCup_296-425 |
| 148169 | pGex-CS-DmCup_296-425-L364AL368A-GB1_Z | Bacterial Expression of DmCup_296-425-L364AL368A |
| 148170 | pGex-CS-DmCup_296-425-Y327AL332A-GB1_Z | Bacterial Expression of DmCup_296-425-Y327AL332A |
| 148171 | pGex-CS-DmCup_296-425-YLLLtoAAAA-GB1_Z | Bacterial Expression of DmCup_296-425-YLLLtoAAAA |
| 148172 | pnEA-NpH-DmeIF4E_69-248_Z | Bacterial Expression of DmeIF4E_69-248 |
| 148173 | pnEA-NpH-DmeIF4E_69-248-I96AI112A_Z | Bacterial Expression of DmeIF4E_69-248-I96AI112A |
| 148174 | pnEA-NpM-Dm4E-T_9-21-GB1_Z | Bacterial Expression of Dm4E-T_9-21 |
| 148175 | pnEA-NpM-Dm4E-T_9-44-GB1_Z | Bacterial Expression of Dm4E-T_9-44 |
| 148176 | pnEA-NpM-Dm4E-T_21-44-GB1_Z | Bacterial Expression of Dm4E-T_21-44 |
| 148177 | pnEA-NpM-DmCup_342-376-GB1_Z | Bacterial Expression of DmCup_342-376 |
| 148178 | pnEA-NpM-DmThor_50-63-GB1_Z | Bacterial Expression of DmThor_50-63 |
| 148179 | pnEA-NpM-DmThor_50-83_Z | Bacterial Expression of DmThor_50-83 |
| 148180 | pnEA-NpM-DmThor_50-83-GB1_Z | Bacterial Expression of DmThor_50-83 |
| 148181 | pnEA-NpM-DmThor_63-83-GB1_Z | Bacterial Expression of DmThor_63-83 |
| 148182 | pnEA-NvG-Dm4E-T_1-58_Z | Bacterial Expression of Dm4E-T_1-58 |
| 148183 | pnEA-NvG-DmeIF4G_578-650_Z | Bacterial Expression of DmeIF4G_578-650 |
| 148184 | pnEA-NvG-DmThor_Z | Bacterial Expression of DmThor |
| 148185 | pnEA-NvM-Dm4E-T_1-58_Z | Bacterial Expression of Dm4E-T_1-58 |
| 148186 | pnEA-NvM-Dm4E-T_1-58-del28-43_Z | Bacterial Expression of Dm4E-T_1-58-del28-43 |
| 148187 | pnEA-NvM-Dm4E-T_1-58-Y10AL15A_Z | Bacterial Expression of Dm4E-T_1-58-Y10AL15A |
| 148188 | pnEA-NvM-DmThor_Z | Bacterial Expression of DmThor |
| 148189 | pnEA-NvM-DmThor-CLL78AAA_Z | Bacterial Expression of DmThor-CLL78AAA |
| 148190 | pnEA-NvM-DmThor-del76-84_Z | Bacterial Expression of DmThor-del76-84 |
| 148191 | pnEA-NvM-DmThor-del76-84-Y54AM59A_Z | Bacterial Expression of DmThor-del76-84-Y54AM59A |
| 148192 | pnEA-NvM-DmThor-GB1_Z | Bacterial Expression of DmThor |
| 148193 | pnEA-NvM-DmThor-Y54AM59A_Z | Bacterial Expression of DmThor-Y54AM59A |
| 148194 | pnEA-NvM-DmThor-YMCLLtoAAAAA_Z | Bacterial Expression of DmThor-YMCLLtoAAAAA |
| 148195 | pnEK-NvH-DmeIF4E_Z | Bacterial Expression of DmeIF4E |
| 148196 | pnEK-NvH-DmeIF4E-I96AI112A_Z | Bacterial Expression of DmeIF4E-I96AI112A |
| 148197 | pnEK-NvSHN-DmeF4E-I96AI112A_Z | Bacterial Expression of DmeIF4E-I96AI112A |
| 148198 | pnEK-NvSHN-DmeIF4E_Z | Bacterial Expression of DmeIF4E |
| 148283 | pnEA-NpM-Dm4E-T_9-44-Y10AL15A-GB1_AA | Bacterial Expression of Dm4E-T_9-44-Y10AL15A |
| 148284 | pnEA-NpM-DmThor_50-83-Y54AM59A-GB1_AA | Bacterial Expression of DmThor_50-83-Y54AM59A |