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Mextli proteins use both canonical bipartite and novel tripartite binding modes to form eIF4E complexes that display differential sensitivity to 4E-BP regulation.
Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, Weichenrieder O, Igreja C, Izaurralde E
Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. Epub 2015 Aug 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
79262pAc5.1B-EGFP-DmMextliExpresses EGFP-tagged DmMextli in S2 cells
148337pAc5.1B-EGFP-DmMextli_577-620_ABInsect Expression of DmMextli_577-520
148338pAc5.1B-EGFP-DmMextli_577-640_ABInsect Expression of DmMextli_577-640
148339pAc5.1B-lambdaN-HA-DmeIF4E-N110EH111E-siRNAres_ABInsect Expression of DmeIF4E-N110EH111E-siRNAres
148340pAc5.1B-lambdaN-HA-DmMextli-F625E_ABInsect Expression of DmMextli-F625E
148341pAc5.1B-lambdaN-HA-DmMextli-F625EY630E_ABInsect Expression of DmMextli-F625EY630E
148342pAc5.1B-lambdaN-HA-DmMextli-I612AL613A_ABInsect Expression of DmMextli-I612AL613A
148343pAc5.1B-lambdaN-HA-DmMextli-L598D_ABInsect Expression of DmMextli-L598D
148344pAc5.1B-lambdaN-HA-DmMextli-M605D_ABInsect Expression of DmMextli-M605D
148345pAc5.1B-lambdaN-HA-DmMextli-W596D_ABInsect Expression of DmMextli-W596D
148346pAc5.1B-lambdaN-HA-DmMextli-W602D_ABInsect Expression of DmMextli-W602D
148347pAc5.1B-lambdaN-HA-DmMextli-Y581AL587AL596A_ABInsect Expression of DmMextli-Y581AL587AL596A
148348pAc5.1B-lambdaN-HA-DmMextli-YLLILtoAAAAA_ABInsect Expression of DmMextli-YLLILtoAAAAA
148349pnEA-NpM-CeeIF4G_315-491opt_ABBacterial Expression of CeeIF4G_315-491opt
148350pnEA-NpM-CeMextli_471-507opt_ABBacterial Expression of CeMextli_471-507opt
148351pnEA-NpM-CeMextli_471-507opt-GB1_ABBacterial Expression of CeMextli_471-507opt
148352pnEA-NpM-CeMextli_471-507opt-I497DI504D-GB1_ABBacterial Expression of CeMextli_471-507opt-I497DI504D
148353pnEA-NpM-CeMextli_471-507opt-M493D-GB1_ABBacterial Expression of CeMextli_471-507opt-M493D
148354pnEA-NpM-CeMextli_471-507opt-Y479AL484AM485A-GB1_ABBacterial Expression of CeMextli_471-507opt-Y479AL484AM485A
148355pnEA-NpM-DmMextli_577-620_ABBacterial Expression of DmMextli_577-620
148356pnEA-NpM-DmMextli_577-620-GB1_ABBacterial Expression of DmMextli_577-620
148357pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_ABBacterial Expression of DmMextli_577-620-I583RS590R
148358pnEA-NpM-DmMextli_577-620-I612AL613A-GB1_ABBacterial Expression of DmMextli_577-620-I612AL613A
148359pnEA-NpM-DmMextli_577-620-Y581AL586AL587A-GB1_ABBacterial Expression of DmMextli_577-620-Y581AL586AL587A
148360pnEA-NpM-DmMextli_577-640_ABBacterial Expression of DmMextli_577-640
148361pnEA-NpM-DmMextli_577-640-F625EL631E-GB1_ABBacterial Expression of DmMextli_577-640-F625EL631E
148362pnEA-NpM-DmMextli_577-640-GB1_ABBacterial Expression of DmMextli_577-640
148363pnEA-NpM-DmMextli_577-640-I612AL613A-GB1_ABBacterial Expression of DmMextli_577-640-I612AL613A
148364pnEA-NpM-DmMextli_577-640-M605D-GB1_ABBacterial Expression of DmMextli_577-640-M605D
148365pnEA-NpM-DmMextli_577-640-Y581AL586AL587A-GB1_ABBacterial Expression of DmMextli_577-640-Y581AL586AL587A
148366pnEA-NpM-DmMextli_577-640-YLLILtoAAAAA-GB1_ABBacterial Expression of DmMextli_577-640-YLLILtoAAAAA
148367pnYC-NpH-CeeIF4E3_1-215opt_ABBacterial Expression of CeeIF4E3_1-215
148368pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_ABBacterial Expression of CeeIF4E3_1-215-V58AI74A
148369pnYC-NpH-CeeIF4E3_30-215opt_ABBacterial Expression of CeeIF4E3_30-215opt

Antibodies from Article