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An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, Arnold DB
Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79869pCAG_EGFP-GFE3Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.
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79870pCAG_EGFP-RandE3Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
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79871pAAV-Ef1a-DIO_EGFP-GFE3For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.
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79872pAAV-Ef1a-DIO_EGFP-RandE3For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.
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