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Design and implementation of a biomolecular concentration tracker.
Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. Epub 2014 May 15. PubMed Article

Plasmids from Article

ID Plasmid Purpose
80392pWW2149Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains.
80393pVH028Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
80394pVH001Contains aTc inducible Scaffold-mCherry-AAV and TetR genes
80395pVH011Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.
80396pVH002pTet-Scaffold with no fluorescent protein. Used as a control for autofluorescence
80397pVH003Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments
80398pVH015Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots
80399pVH009Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.
80400pVH016No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).
80401pVH004Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

Antibodies from Article