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ALIX-CHMP4 interactions in the human ESCRT pathway.
McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29. PubMed Article

Plasmids from Article

ID Plasmid Purpose
80630GST-CHMP4A (pGEX2T(TEV))Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197
80632GST-CHMP4A (1-204) (pGEX2T(TEV))Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198
80633GST-CHMP4A (205-222) (pGEX2T(TEV))Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60
80634GST-CHMP4B (205-224) (pGEX2T(TEV))Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201
80635GST-CHMP4C (216-233) (pGEX2T(TEV))Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202
80637GST-CHMP4A (205-222) W220A (pGEX2T(TEV))Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203
80638GST-CHMP4A (205-222) L217A (pGEX2T(TEV))Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204
80639GST-CHMP4A (205-222) L214A (pGEX2T(TEV))Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205
80640GST-CHMP4A (205-222) E209A (pGEX2T(TEV))Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
80641pet16b ALIX Bro1 (1-359)Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428
80642pet151D-TOPO ALIX Bro1-V (1-702)Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

Antibodies from Article