Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments.
Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ
Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392.
PubMed

Plasmids from Article

ID Plasmid Purpose
80760pJAG98-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
80761pBABE-puro-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
80762pBABE-hygro-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
83285pTY07human CENP-A N-terminally labeled with LSSmOrange
83286pJAG98-mTurquoise2-FHA2-AURKB substrate-YPetuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Antibodies from Article