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CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002.
Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger BF
Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 10.1016/j.ymben.2016.07.007.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
82371pGCG26YFP expressed from pJ23119 in the glpK locus with gentamicin resistance
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82372pCas2AdCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA
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82373pCas2BdCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA
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82374pCas2CdCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA
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82375pCas2DdCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG
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82376pCas2EdCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG
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82377pCas2FdCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG
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82378pCas5sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance
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82379pCas7dCas9 expressed from low constituve promoter, c225, in acsA
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82380pCas8dCas9 without a promoter in acsA
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82381pCas13sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance
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82382pCas14sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance
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82383pCas15sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance
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82384pCas27sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance
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82385pCas28sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance
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82386pCas34sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82387pCas35sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82388pCas36sgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82389pCas39sgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82390pCas45sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance
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82391pCas49rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK
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82392pCas52sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82393pCas55sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82394pCas56sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82395pCas57sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82396pCas59sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
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82397pCas61IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK
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82398pCas73Δccm:Km, used to delete ccm operon
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