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Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells.
Kornete M, Marone R, Jeker LT
J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
82661p242_LTJ_1kbCD45.1Template1kb HDR template for murine CD45.1
82662p248_LTJ_2kbCD45.1Template2kb HDR template for murine CD45.1
82663p243_LTJ_4kbCD45.1Template4kb HDR template for murine CD45.1
82664p249_LTJ_1kbFoxp3wtTemplate1kb HDR template for murine wildtype Foxp3
82665p250_LTJ_2kbFoxp3wtTemplate2kb HDR template for murine wildtype Foxp3
82666p213_LTJ_160bpCD90.1Template160bp HDR template for murine CD90.1
82667p214_LTJ_1kbCD90.1Template1kb HDR template for murine CD90.1
82668p229_LTJ_2kbCD90.1Template2kb HDR template for murine CD90.1
82669p230_LTJ_4kbCD90.1Template4kb HDR template for murine CD90.1
82670p184_LTJ_sgRNACD90.2sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.
82671p183_LTJ_sgRNACD90.2_AsgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.
82672p202_LTJ_sgRNACD45.2_R1sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.
82673p204_LTJ_sgRNACD45.2_R2sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.
82674p206_LTJ_sgRNACD45.2_R3sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.
82675p240_LTJ_sgRNAFoxp3K276XsgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.
82676p236_LTJ_sgRNAFoxp3sf/JsgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.
82677p163_LTJ_sgRNACD90.1sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.
104988p350_LTJ_180bpCD90.1Template180bp HDR template for murine CD90.1

Antibodies from Article