Regulation of Polycystin-1 Function by Calmodulin Binding.
Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016. PubMed
Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83451 | pcDNA3 GFP-h-PKD2 | GFP-tagged PC2 for Eukaryotic expression (883) |
| 83452 | pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His | Full-length murine PC1 for Eukaryotic Expression (982) |
| 83453 | pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His | Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037) |
| 83454 | pcDNA4/TO h-PKD1 FLS R4150C-GST | Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850) |
| 83455 | pcDNA4/TO h-PKD1 FLS L4137P-GST | Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848) |
| 83456 | pcDNA4/TO h-PKD1 FLS (4107-4303)-GST | Human PC1-CTp30 expression cassette with a GST-tag (678) |
| 83457 | pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His | Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632) |
| 83458 | pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His | Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629) |
| 83459 | pcDNA4/TO h-PKD1 FLS S4144D-Myc/His | Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514) |
| 83460 | pcDNA4/TO CD16.7-h-PKD1 FLM S4144D | Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513) |
| 83461 | pcDNA4/TO CD16.7-h-PKD1 FLM R4114A | Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503) |
| 83462 | pcDNA4/TO CD16.7-h-PKD1 FLM W4108A | Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502) |
| 83463 | pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169) | Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96) |
| 83465 | pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303) | Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98) |