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Regulation of Polycystin-1 Function by Calmodulin Binding.
Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
PLoS One. 2016 Aug 25;11(8):e0161525. doi: 10.1371/journal.pone.0161525. eCollection 2016.
PubMed

Plasmids from Article

ID Plasmid Purpose  
83451pcDNA3 GFP-h-PKD2GFP-tagged PC2 for Eukaryotic expression (883)
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83452pcDNA4/TO 3xFlag-m-PKD1-2xMyc/HisFull-length murine PC1 for Eukaryotic Expression (982)
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83453pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/HisFull-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)
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83454pcDNA4/TO h-PKD1 FLS R4150C-GSTHuman PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)
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83455pcDNA4/TO h-PKD1 FLS L4137P-GSTHuman PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)
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83456pcDNA4/TO h-PKD1 FLS (4107-4303)-GSTHuman PC1-CTp30 expression cassette with a GST-tag (678)
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83457pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/HisHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)
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83458pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/HisHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)
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83459pcDNA4/TO h-PKD1 FLS S4144D-Myc/HisHuman PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)
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83460pcDNA4/TO CD16.7-h-PKD1 FLM S4144DCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)
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83461pcDNA4/TO CD16.7-h-PKD1 FLM R4114ACytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)
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83462pcDNA4/TO CD16.7-h-PKD1 FLM W4108ACytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)
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83463pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)
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83465pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)
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