DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
Mol Cell. 2016 Sep 1;63(5):840-51. doi: 10.1016/j.molcel.2016.07.027. PubMed Article
Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
Mol Cell. 2016 Sep 1;63(5):840-51. doi: 10.1016/j.molcel.2016.07.027. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81182 | D. vulgaris Cas5c/pHMGWA | Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag |
| 81183 | D. vulgaris Cas8c/pHMGWA | Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag |
| 81184 | D. vulgaris Cas7/pHMGWA | Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag |
| 81185 | D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA | Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c |
| 81186 | D. vulgaris sp2 CRISPR/pACYCDuet-1 | Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli |