Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.
East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA
Nature. 2016 Sep 26. doi: 10.1038/nature19802.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83482 | p2CT-His-MBP-Lbu_C2c2_WT | Bacterial expression of WT L. buccalis C2c2 CRISPR effector. |
| 83483 | p2CT-His-MBP-Lbu_C2c2_R472A_H477A | Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant). |
| 83484 | p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A | Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant). |
| 83485 | p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A | Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant). |
| 83486 | p2CT-His-MBP-Lse_C2c2_WT | Bacterial expression of WT L. seeligeri C2c2 CRISPR effector |
| 83487 | p2CT-His-MBP-Lsh_C2c2_WT | Bacterial expression of WT L. shahii C2c2 CRISPR effector |
| 83494 | p2CT-His-MBP-Lbu_C2c2_R1079A | Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant) |