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Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly.
Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, Sieverding C, Madlung J, Gerken U, Macek B, Antonin W
EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. Epub 2012 Sep 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
85998pET28a SUMO Nup53xl 1-320bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53
85999pET28a MISTICbacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag
86000pSI HA Nup53xlmammalian expression of HA tagged Xenopus laevis Nup53
86001pSI myc Nup53xlmammalian expression of myc tagged Xenopus laevis Nup53

Antibodies from Article