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The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14.
Park JM, Jung CH, Seo M, Otto NM, Grunwald D, Kim KH, Moriarity B, Kim YM, Starker C, Nho RS, Voytas D, Kim DH
Autophagy. 2016;12(3):547-64. doi: 10.1080/15548627.2016.1140293.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86747myc-Atg14N-terminal myc-tagged protein expression in mammaiian cells
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86748HA-Beclin 1N-terminal HA-tagged protein expression in mammaiian cells
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86749HA-Vps34N-terminal HA-tagged protein expression in mammaiian cells
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86750myc-Atg14 (1-200)N-terminal myc-tagged protein expression in mammaiian cells
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86751myc-Atg14 (201-492)N-terminal myc-tagged protein expression in mammaiian cells
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86752myc-Atg14 (1-181)N-terminal myc-tagged protein expression in mammaiian cells
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86753myc-Atg14 (71-181)N-terminal myc-tagged protein expression in mammaiian cells
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86754myc-Atg14 (181-413)N-terminal myc-tagged protein expression in mammaiian cells
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86755myc-Atg14 (396-492)N-terminal myc-tagged protein expression in mammaiian cells
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86756myc-Atg14 S29AN-terminal myc-tagged protein expression in mammaiian cells
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86757myc-Atg14 S29DN-terminal myc-tagged protein expression in mammaiian cells
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