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The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14.
Park JM, Jung CH, Seo M, Otto NM, Grunwald D, Kim KH, Moriarity B, Kim YM, Starker C, Nho RS, Voytas D, Kim DH
Autophagy. 2016;12(3):547-64. doi: 10.1080/15548627.2016.1140293.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
86747myc-Atg14N-terminal myc-tagged protein expression in mammalian cells
86748HA-Beclin 1N-terminal HA-tagged protein expression in mammalian cells
86749HA-Vps34N-terminal HA-tagged protein expression in mammalian cells
86750myc-Atg14 (1-200)N-terminal myc-tagged protein expression in mammalian cells
86751myc-Atg14 (201-492)N-terminal myc-tagged protein expression in mammalian cells
86752myc-Atg14 (1-181)N-terminal myc-tagged protein expression in mammalian cells
86753myc-Atg14 (71-181)N-terminal myc-tagged protein expression in mammalian cells
86754myc-Atg14 (181-413)N-terminal myc-tagged protein expression in mammalian cells
86755myc-Atg14 (396-492)N-terminal myc-tagged protein expression in mammalian cells
86756myc-Atg14 S29AN-terminal myc-tagged protein expression in mammalian cells
86757myc-Atg14 S29DN-terminal myc-tagged protein expression in mammalian cells

Antibodies from Article