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Crystal structure of the 5hmC specific endonuclease PvuRts1I.
Kazrani AA, Kowalska M, Czapinska H, Bochtler M
Nucleic Acids Res. 2014 May;42(9):5929-36. doi: 10.1093/nar/gku186. Epub 2014 Mar 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
86887pET15b-PvuRts1Iexpress N-term His tagged PvuRts1I in ER2566 strain
86888pET28a-PvuRts1Iexpress C-term His tagged PvuRts1I in ER2566 strain
86890pET15b-D70A-PvuRts1Iexpress D70A variant PvuRts1I in ER2566 strain
86891pET15b-R208A-PvuRts1Iexpress R208A variant PvuRts1I in ER2566 strain
86892pET15b-D57A-PvuRts1Iexpress D57A variant PvuRts1I in ER2566 strain
86894pET15b-H74A-PvuRts1Iexpress H74A variant PvuRts1I in ER2566 strain
86895pET15b-E71A-PvuRts1Iexpress E71A variant PvuRts1I in ER2566 strain
86896pET15b-E228A-PvuRts1Iexpress E228A variant PvuRts1I in ER2566 strain
86897pET15b-E20A-PvuRts1Iexpress E20A variant PvuRts1I in ER2566 strain
86898pET15b-W215A-PvuRts1Iexpress W215A variant PvuRts1I in ER2566 strain
86900pTXB1-AbaSFIExpress CBD and intein tag containing AbaSI enzyme full length

Antibodies from Article