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Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.
Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68369EPB104contains human U6 promoter
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68370U6GRNAgRNA scaffold with hU6 promoter
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68371CAS9PBKSCbAkt promoter driven Cas9-2A-GFP (from px458)
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Antibodies from Article