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Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.
Zamora CY, Schocker NS, Chang MM, Imperiali B
Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89708GST-PglF130Expresses truncated PglF from C. jejuni with N-terminal GST tag
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89711T7-WbpB-His6Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag
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89712T7-WbpE-His6Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag
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89713T7-WbpD-His6Expresses WbpD from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag
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89714T7-AglK-HisExpresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.
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89723T7-PseB-His6Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag
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89724His8-TEV-PseCExpresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site
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89725His8-TEV-PseHExpresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site
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89726T7-AglC-His6Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.
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Antibodies from Article