Efficient sortase-mediated N-terminal labeling of TEV protease cleaved recombinant proteins.
Sarpong K, Bose R
Anal Biochem. 2017 Mar 15;521:55-58. doi: 10.1016/j.ab.2017.01.008. Epub 2017 Jan 11. PubMed Article
Sarpong K, Bose R
Anal Biochem. 2017 Mar 15;521:55-58. doi: 10.1016/j.ab.2017.01.008. Epub 2017 Jan 11. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
86962 | pET30b-5M SrtA with TEV site | Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag |