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A genetic system to study Plasmodium falciparum protein function.
Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85789pSLI-2xFKBP-GFP(1463000)P. falciparum integration plasmid to fuse endogenous genes with tags using SLI
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85790pSLI-sandwich(0807600)P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI
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85791pSLI-TGD(1463000)P. falciparum integration plasmid to disrupt genes using SLI
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85792pSLI-N-GFP-2xFKBP-loxP(K13)P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI
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85793pSLI-N-sandwich-loxP(K13)P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI
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85795p1xNLS-FRB-mCherry-hsp86-BSDExpression of 1xNLS mislocaliser in P. falciparum
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85796pLyn-FRB-mCherry-nmd3-BSDExpression of PPM mislocaliser in P. falciparum
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85797pSkipFloxP. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide
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