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Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.
Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B
Proc Natl Acad Sci U S A. 2017 Apr 24. pii: 201616395. doi: 10.1073/pnas.1616395114.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89238pMBPCas2/3Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion
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89240pCas1_Cas2/3Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
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89246pAcrF3Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).
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