Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.
Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B
Proc Natl Acad Sci U S A. 2017 Apr 24. pii: 201616395. doi: 10.1073/pnas.1616395114. PubMed Article
Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B
Proc Natl Acad Sci U S A. 2017 Apr 24. pii: 201616395. doi: 10.1073/pnas.1616395114. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89238 | pMBPCas2/3 | Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion |
| 89240 | pCas1_Cas2/3 | Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1 |
| 89246 | pAcrF3 | Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5). |