enChIP systems using different CRISPR orthologues and epitope tags.
Fujita T, Yuno M, Fujii H
BMC Res Notes. 2018 Feb 27;11(1):154. doi: 10.1186/s13104-018-3262-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92220 | pLenti_dCas9-2xAM | Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites |
| 92221 | pLenti_dCas9-2xAM_hIRF-1 | Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter |
| 98041 | Sa-dCas9-NLS-3xFLAG/pcDNA3.1 | Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest |
| 98134 | hIRF-1 gRNA #409 (Sa) | Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system |
| 105283 | hIRF-1 gRNA #351 (Sa) | Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system |