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A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
Zhang XR, He JB, Wang YZ, Du LL
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98698pDB4279A control plasmid containing the intact bsdMX marker
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98699pDB4280A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
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98700pDB4281A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
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98701pDB4282A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system
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98702pDB4283A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
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